16S rRNA metagenomic profiling of red amaranth grown organically with different composts and soils.

In recent years organic food is gaining popularity as it is believed to promote better human health and improve soil sustainability, but there are apprehensions about pathogens in organic produces. This study was designed to understand the effect of different composts and soils on the status of the microbiome present in organically grown leafy vegetables. 16S rRNA metagenomic profiling of the leaves was done, and data were analyzed. It was found that by adding composts, the OTU of the microbiome in the organic produce was higher than in the conventional produce. The beneficial genera identified across the samples included plant growth promoters (Achromobacter, Paenibacillus, Pseudomonas, Sphingobacterium) and probiotics (Lactobacillus), which were higher in the organic produce. Some pathogenic genera, viz., plant pathogenic bacteria (Cellvibrio, Georgenia) and human pathogenic bacteria (Corynebacterium, Acinetobacter, Streptococcus, Streptomyces) were also found but with relatively low counts in the organic produce. Thus, the present study highlights that organic produce has lesser pathogen contamination than the conventional produce. KEY POINTS: • 16S rRNA metagenomics profiling done for organic red amaranth cultivar • Microbial richness varied with respect to the soil and compost type used • The ratio of beneficial to pathogenic genera improves with the addition of compost.

Fluoride in groundwater of industrial town of Sonbhadra district, Uttar Pradesh, India: probable release mechanism and potential health risk assessment.

In the selected study region of Sonbhadra district, coal burning and mining activities are dominant. Previous studies reported F contamination in very few groundwater samples of this region. A detailed study is required to estimate the fluoride in groundwater of this area. Hence, a total of 128 groundwater samples were collected during post- and pre-monsoon seasons in the year 2017 to estimate the F-, its geochemistry, and health risk assessment from Renukoot and Anpara industrial clusters of Sonbhadra district, Uttar Pradesh, India. The pH of groundwater samples varied from slightly acidic to alkaline during both seasons. Almost all the major cations (Ca2+, Mg2+, Na+, and K+) and major anions (HCO3-, Cl-, SO42-, and F-) values in groundwater samples of both clusters were found within the permissible limit of World Health Organization (WHO) and Beauro of Indian standards except F- in both seasons. The scatter plots of F- with Ca2+, Na+, HCO3-, and pH are used to explain the release mechanism of fluoride in groundwater. Saturation indices (SI) calcite versus SI fluorite and SI dolomite versus SI fluorite plots of both clusters used to check the involvement of these minerals in fluoride enrichment of aquifers. F- contamination in groundwater due to coal burning in coal mining and thermal power plant dominated region is discussed globally and locally both. The non-carcinogenic health risk due to consumption of fluoride-contaminated water is estimated by using target hazard quotient (THQ). THQ values of F- showed that children are at high risk than adults in both clusters of the study area during both seasons. Pictorial representation is used to show the dental fluorosis cases in children of the study region.

Formation and characterization of leaf waste into organic compost.

In solid waste management, pollution-free disposal of leaf waste in urban areas is still not standardized and adopted. According to the World Bank report, 57% of wastes generated in South East Asia are consisted of food and green waste, which can be recycled into valuable bio-compost. The present study shows a method of leaf litter waste management by composting it using essential microbe (EM) method. Different parameters, such as pH, electrical conductivity, macronutrients, micronutrients, and potentially toxic elements (PTE) were measured at zero to 50 days of composting using appropriate methods. The microbial composting was shown to mature within 20 to 40 days, and its maturity could be evaluated by the attainment of stable pH (8), electrical conductivity (0.9 mS/cm), and C:N ratio ≥ 20. The analysis was also performed on other bio-composts viz. kitchen waste compost, vermicompost, cow dung manure, municipal organic waste compost, and neem cake compost. The fertility index (FI) was evaluated based on six parameters viz. total carbon, total nitrogen, N ratio, phosphorus, potassium, and sulphur contents. The PTE values were used to calculate their clean index (CI). The results showed that leaf waste compost has a higher fertility index (FI = 4.06) than other bio-composts, except the neem cake compost (FI = 4.44). The clean index of the leaf waste compost (CI = 4.38) was also higher than other bio-composts. This indicates that leaf waste compost is a valuable bio-resource with high nutritive value and low PTE contamination, with a favourable prospective to be used in organic farming.

Potentially toxic elements in soil and road dust around Sonbhadra industrial region, Uttar Pradesh, India: Source apportionment and health risk assessment.

Potentially toxic elements (PTEs) are directly linked with various kinds of adverse health issues. Available reports related to symptoms of mercury contamination in the local population of the study region motivated us to carry out this work in detail. To estimate potentially toxic elements (As, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Zn) contamination status, a total of 48 samples of soil & road dust from industrial clusters were collected and analyzed for source identification and human health risk assessment in the Sonbhadra region of Uttar Pradesh, India. As per upper continental crust (UCC) for soil and road dust, the highest increment of As value in Obra and Hg value in Anpara was observed. The value of Hg exceeded the background value by 6.5 and 12.25 times in soil and 5 and 11.5 times in road dust of Obra and Anpara clusters, respectively. Contamination factor (CF) and Enrichment factor (EF) value in soil and road dust showed very strong contamination and significant enrichment of Hg whereas moderate contamination and moderate enrichment of As were observed in both the clusters. The hazard quotient (HQ) value of potentially toxic elements in soil and road dust of Obra and Anpara were found <1 for three pathways in adults and children, except Fe for ingestion pathway for children in both clusters. The HQ value for adults was observed to be low compared to children. Cancer risk associated with potentially toxic elements in soil and road dust for both clusters were found safe (under the guideline 10-4-10-6) in adult and children instances for three pathways. Principal component analysis (PCA) justified the metal content in soil and road dust controlled by the mixed type of both natural and anthropogenic sources.

Trace elements contamination in groundwater and associated human health risk in the industrial region of southern Sonbhadra, Uttar Pradesh, India.

The present study assesses the pollution load of the groundwater with reference to the trace elements (i.e. As, Hg, Cd, Cr, Cu, Fe, Mn, Zn, Ni, Co and Pb) and the potential health risk by its consumption for the residents of Obra, Renukoot and Anpara industrial clusters of Southern Sonbhadra, Uttar Pradesh, India. For this, 220 groundwater samples were collected during post- and premonsoon seasons in 2015. pH varied from slightly acidic to alkaline in both the seasons. Geochemical analysis of the area showed that all the three clusters are severely contaminated with Fe, Pb, Cd, Cr, As and Hg during both the seasons. High concentration of heavy metals indicates that groundwater was contaminated with natural as well as anthropogenic sources. For all the three clusters, the mean values of heavy metal pollution index were found above the critical index in both the seasons with Anpara in lead. For the majority of groundwater samples across the clusters during both the seasons, substantial non-cancer health risk was observed due to target hazard quotient values of Cr, Cd, As, Pb and Hg higher than unity. The hazard index value for children was very high compared to adults which means that children are more susceptible to health impairment in terms of non-carcinogenic health risk. Carcinogenic risk was higher for adults than children in the entire study area.